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Development of an On‐Line Electrochemical Biosensor for Glucose Determination in Rat Brain Using Microdialysis Sampling
Author(s) -
Pravda Miloslav,
Kauffmann JeanMichel,
Michotte Yvette
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200008)12:12<912::aid-elan912>3.0.co;2-z
Subject(s) - microdialysis , biosensor , amperometry , glucose oxidase , chemistry , chromatography , flow injection analysis , electrode , extracellular , analytical chemistry (journal) , electrochemistry , detection limit , biochemistry
A biosensor for on‐line monitoring of glucose in brain dialysates was developed. The biosensor was generated in the cross‐flow amperometric cell by potentiostatic electropolymerization of phenol (50 mM) in the presence of glucose oxidase (935 U/mL) at +600 mV ( vs . Ag/AgCl) at a flow‐rate of 5 µL/min at the Pt electrode. The polyphenol film ensured low interferences of ascorbate. The on‐line set‐up was mathematically described and the model was used to calculate the extracellular concentration of glucose in the striatum of an anesthetized rat. The concentration of glucose was found to be 1.4±0.5 mM and 0.9±0.2 mM in two independent experiments.