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Electrochemical Studies of the Hemin‐DNA Interaction
Author(s) -
Qu Feng,
Zhu Zhiwei,
Li NanQiang
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200007)12:11<831::aid-elan831>3.0.co;2-0
Subject(s) - hemin , porphyrin , electrochemistry , dna , chemistry , intercalation (chemistry) , deoxyribozyme , inorganic chemistry , dimer , steric effects , electrode , photochemistry , combinatorial chemistry , heme , organic chemistry , biochemistry , enzyme
An electrochemical study of the interactions of hemin with DNA on a hanging mercury drop electrode (in ammonia buffer solution) is described. Adding DNA in hemin solution resulted in decrease of both the hemin reduction peak and the catalytic hydrogen wave. Electrochemically nonactive hemin‐DNA complexes are formed due to hemin intercalated into DNA. Then the equilibrium concentration of hemin in solution decreases. In this article, we give some support to the fact that hemin, as a dimer under our experiment, with a large steric structure and anionic substitute on porphyrin plane, shows the abilities to intercalate into DNA.