Premium
Simultaneous Determination of 2‐Aminothiazole, 2‐Aminobenzothiazole and 2‐Mercaptobenzothiazole by Capillary Electrophoresis with End‐Column Amperometric Detection
Author(s) -
You Tianyan,
Wang Xiaoqing,
Yang Xiurong,
Wang Erkang
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200007)12:11<821::aid-elan821>3.0.co;2-4
Subject(s) - capillary electrophoresis , detection limit , amperometry , chemistry , chromatography , phosphate buffered saline , analytical chemistry (journal) , linear range , electrophoresis , buffer (optical fiber) , buffer solution , capillary action , electrode , electrochemistry , materials science , telecommunications , computer science , composite material
Capillary electrophoresis with amperometric detection is evaluated for the simultaneous determination of 2‐aminothiazole (A), 2‐aminobenzothiazole (AB), 2‐mercaptobenzothiazole (AM). The cyclic voltammogram, hydrodynamic voltammogram, effect of pH, concentration of buffer and separation voltage on the separation and the detection were studied. The conditions were optimized as follows: 50 mM phosphate buffer, pH 6.0, 2s at 17.5 kV sample injection, separation at 17.5 kV, 1.2 V as detection potential. The method provided low detection limit as 0.5 µM, 0.05 µM and 0.01 µM, wide linear range 2–200 µM, 10–200 µM and 0.025–100 µM for A, AB, and AM, respectively. The variations in peak current and migration time for 15 consecutive injections of a standard containing 5 µM each compound were 3.7, 2.1, and 3.9 %, and 1.2, 0.8, and 1.2 %, for A, AB and AM, respectively. This method was employed to analyze river water.