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A Sensitive Electrochemical Protein Quantification Method
Author(s) -
Schwarz Alexandra,
Bagel Olivier,
Girault Hubert H.
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200007)12:11<811::aid-elan811>3.0.co;2-8
Subject(s) - biuret test , chemistry , bovine serum albumin , copper , detection limit , anodic stripping voltammetry , differential pulse voltammetry , chromatography , electrochemistry , bradford protein assay , voltammetry , copper protein , bicinchoninic acid assay , electrode , cyclic voltammetry , biochemistry , urea , organic chemistry
This article presents a sensitive electrochemical method for the quantification of proteins. The assay used the biuret reaction, which is based on the complexation of proteins with copper ions to form a copper‐protein complex. In the present approach, once copper ions were complexed with the protein, they were released by an acidic treatment and simultaneously separated by ultracentrifugation from the protein sample. This allowed differential pulse anodic stripping voltammetry (DPASV) of Cu 2+ on carbon rotogravure printed electrodes (CARPEs). A nM detection limit for bovine serum albumin (BSA) was found with this method. The kinetics of this assay were tested and it resulted that the total assay can be carried out in 27 min, including incubation, washing steps and detection.