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Sensitivity of Electrochemically Nanostructured Carbon Fiber Ultramicroelectrodes in the Determination of Adenosine
Author(s) -
ElNour Kholoud Abou,
BrajterToth Anna
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200007)12:11<805::aid-elan805>3.0.co;2-x
Subject(s) - ultramicroelectrode , adenosine , electrochemistry , electrode , horizontal scan rate , chemistry , fiber , analytical chemistry (journal) , materials science , cyclic voltammetry , chromatography , organic chemistry , biochemistry
A direct and fast voltammetric method of determination of adenosine, with a novel carbon fiber ultramicroelectrode (CFE), is demonstrated here. To achieve high sensitivity required in biological determinations, before the determination of adenosine, carbon fiber ultramicro electrodes were activated by electrochemical pretreatment. The electrochemical activation process generates a nanostructured carbon fiber surface which is stable. The high stability of the active surface, and the high reproducibility of the activation process, leads to reproducible and sensitive fast scan voltammetric (FSV) determinations of adenosine. Adenosine sensitivity was determined in 7×10 –2 M phosphate buffer, pH 7.4, at a scan rate of 500 V s –1 . The potential window in the determinations was from –1.0 to 1.5 V (vs. SCE). A high sensitivity of the new method of adenosine determinations was achieved under physiological conditions.