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A Minisensor for the Rapid Screening of Acesulfame‐K, Cyclamate, and Saccharin Based on Surface‐Stabilized Bilayer Lipid Membranes
Author(s) -
Nikolelis Dimitrios P.,
Pantoulias Spyros
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200006)12:10<786::aid-elan786>3.0.co;2-3
Subject(s) - chemistry , membrane , bilayer , lipid bilayer , phosphatidylcholine , saccharin , electrochemistry , electrolyte , chromatography , chemical engineering , analytical chemistry (journal) , electrode , phospholipid , biochemistry , medicine , engineering , endocrinology
This work describes an electrochemical technique that is suitable for the rapid and sensitive screening of the sweeteners acesulfame‐K, cyclamate and saccharin based on surface‐stabilized bilayer lipid membranes (s‐BLMs) composed from egg phosphatidylcholine (PC). The interactions of the sweeteners with s‐BLMs which produced a reproducible electrochemical ion current signal increase appeared within a few s after exposure of the membranes to the sweetener. The current signal increases were related to the concentration of the sweetener in bulk solution in the micromolar range. The present lipid film based sensor provided fast response (i.e., on the order of a few s) to alterations of acesulfame‐K, cyclamate and saccharin concentration (1 to 15, 10 to 160 and 0.4 to 7 μM) in electrolyte solution. The electrochemical transduction of the interactions of these artificial sweeteners with s‐BLMs was applied in the determination of these compounds in granulated sugar substitute products by using the present minisensor.