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Electrochemical Diagnostic Strip Device for Total Cholesterol and Its Subfractions
Author(s) -
Foster Robert,
Cassidy John,
O'Donoghue Eilish
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200005)12:9<716::aid-elan716>3.0.co;2-s
Subject(s) - amperometry , cholesterol oxidase , cholesterol , electrode , chemistry , ferrocyanide , electrochemistry , linear range , materials science , chromatography , detection limit , biochemistry
This article describes a novel patented electrochemical diagnostic device for determining total cholesterol and high density lipoprotein cholesterol, HDL intended for use in a doctor's office. The device comprises a feeder strip which transfers the sample to the detection zone where the detection is amperometric with ferrocyanide as mediator for the cholesterol/cholesterol oxidase reaction. In order to determine HDL, the other cholesterol subfractions were precipitated and a fibrous disk used to filter out the precipitate. Responses for total cholesterol in the range 2.81 mM to 13.0 mM compare well with a standard method and linear calibration plots have been obtained for cholesterol and HDL. HDL has a linear response between 0.76 mM and 2.3 mM. The device forms a model for a the development of a manufacturable prototype based on screen printed electrodes.