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Rubbed Films of Functionalized Bovine Serum Albumin as Substrates for the Imaging of Protein–Receptor Interactions Using Liquid Crystals
Author(s) -
Kim S.R.,
Abbott N. L.
Publication year - 2001
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/1521-4095(200110)13:19<1445::aid-adma1445>3.0.co;2-9
Subject(s) - rubbing , polarizer , bovine serum albumin , materials science , liquid crystal , covalent bond , biophysics , crystallography , chromatography , optics , organic chemistry , composite material , optoelectronics , chemistry , birefringence , biology , physics
Rubbing is an essential pre‐treatment for covalently immobilized, receptor‐functionalized films of bovine serum albumin to function in liquid‐crystal (LC) imaged protein chips. Only on the rubbed film will LCs form a nematic phase whose disruption by docking target protein is specific and quantifiable using polarized light. The Figure shows the appearance at two anti‐biotin IgG concentrations, the polarizer aligned with the direction of rubbing (left) and shifted by 45° (right).