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Human Low Density Lipoprotein (LDL) and Human Serum Albumin (HSA) Co‐Adsorption Onto the C18‐Silica Gradient Surface
Author(s) -
Hlady V.,
Ho C.H.
Publication year - 2001
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/1521-4052(200102)32:2<185::aid-mawe185>3.0.co;2-w
Subject(s) - adsorption , chemistry , human serum albumin , kinetics , monolayer , desorption , chromatography , low density lipoprotein , organic chemistry , biochemistry , cholesterol , physics , quantum mechanics
Co‐adsorption kinetics of human low density lipoprotein (LDL) and serum albumin (HSA) on hydrophilic/hydrophobic gradient silica surface were studied using Total Internal Reflection Fluorescence (TIRF) and autoradiography. Two experimental systems were examined: (1) fluorescein‐labeled LDL (FITC‐LDL) adsorption from a FITC‐LDL + HSA solution mixture onto the octadecyldimethylsilyl (C18)‐silica gradient surface, and (2) the FITC‐LDL adsorption onto the HSA pre‐adsorbed on the C18‐silica gradient surface. Experiments with fluorescein‐labeled albumin (FITC‐HSA) and unlabeled LDL have been performed in parallel. The adsorption kinetics of FITC‐LDL onto the hydrophilic silica was found to be transport‐limited and not affected by co‐adsorption of HSA. A slower adsorption kinetics of lipoprotein onto the silica with pre‐adsorbed HSA layer resulted from a slow appearance of LDL binding sites exposed by the process of HSA desorption. In the region of increasing surface density of C18 groups, the FITC‐LDL adsorption rate fell below the transport‐limited adsorption rate, except in the very early adsorption times. Pre‐adsorption of HSA onto the C18‐silica gradient region resulted in a significant decrease of both the FITC‐LDL adsorption rate and adsorbed amount. The lowest FITC‐LDL adsorption was found in the region of C18 self‐assembled monolayer, where the pre‐adsorbed HSA layer almost completely eliminated lipoprotein binding.