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Reduction of Leukocyte/Endothelial Cell Interaction Induced by Extracorporal Circulation with the Use of a Coated Tube System
Author(s) -
Kamler M.,
Chatterjee T.,
Trojansky M.,
Gebhard M.M.,
Hagl S.,
Jakob H.,
Stemberger A.
Publication year - 2001
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/1521-4052(200102)32:2<154::aid-mawe154>3.0.co;2-b
Subject(s) - microcirculation , prostacyclin , chemistry , intravital microscopy , endothelial stem cell , in vivo , medicine , biology , in vitro , microbiology and biotechnology , biochemistry
The clinical complications of Extracorporal Circulation (ECC) have been linked to disturbances in the microcirculation. In previous experiments we found in vivo an increased Leukocyte/Endothelial (L/E) cell interaction following ECC. As a therapeutical approach to prevent these deleterious effects a new agent, incorporating Hirudin and Prostacyclin, to coate the tubing system was used. Intravital fluorescence microscopy was used on the dorsal skinfold chamber preparation in syrian golden hamsters. ECC was introduced via a micro‐rollerpump (1 ml/min) and a 60 cm silicon tube (1 mm inner diameter) shunted between the carotid artery and the jugular vein. Experiments were performed in chronically instrumented, awake animals (age: 10–14 weeks, weight: 65–75 g). Control tubes were uncoated, for the experiment a PEG‐Hirudin‐Iloprost® coating was used. Isovolemic ECC for 20 min resulted in an increase in rolling (BL: 9 % ± 2; after 4 h: 36 %* ± 5; mean ± SD, *p < 0.05) and adherent leukocytes (BL: 24 ± 26; after 4 h: 260* ± 51; mean ± SD; p < 0.05) in postcapillary venules. The use of the coated tube system resulted in a less pronounced induction of leukocyte/endothelial cell interaction (Roller: BL: 9 % ± 3; 4 h: 24 %* ± 12; mean ± SD, *p < 0.05. Sticker: BL: 28 ± 25; 4 h: 139 ± 111; mean ± SD; p < 0.05). Microhemodynamic parameters and functional capillary density were not significantly affected. Arterial blood pressure and heart rate were stable. L/E interaction in the microcirculation has been established as an indicator of the systemic activation induced by blood contact to synthetic surfaces during ECC. Coating the extracorporal circuit reduced the increase in L/E interaction probably as a result of an attenuated activation of the coagulation‐fibrinolytic system including a reduced platelet activation.