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Temperature effect in the production of multiple xylanases by Aspergillus fumigatus
Author(s) -
Lenartovicz Veridiana,
Marques De Souza Cristina Giatti,
Moreira Fabiana Guillen,
Peralta Rosane Marina
Publication year - 2002
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/1521-4028(200212)42:6<388::aid-jobm388>3.0.co;2-h
Subject(s) - xylanase , aspergillus fumigatus , chemistry , enzyme , food science , strain (injury) , isozyme , fungi imperfecti , aspergillus , biochemistry , microbiology and biotechnology , biology , anatomy
This work has evaluated the temperature effect in the production of multiple xylanases by a locally isolated strain of Aspergillus fumigatus Fresenius . Three isoenzymes, identified as xylanases I, II, and III with apparent molecular weight of 45.7 KDa, 39.8 KDa and 18.2 KDa, respectively, were produced in cultures developed at 30 °C and at 42 °C. The pattern of distribution of xylanase activity among the three isoenzymes was greatly affected by the growth temperature: at 30 °C, the total xylanase activity was distributed homogeneously among the three enzymes, while at 42 °C, the total xylanase activity was mainly due to the fractions with the highest MW (I and II) and the xylanase III was a minor component.