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Occurrence of crude oil degrading bacteria in gasoline and diesel station soils
Author(s) -
Rahman Kaja Sheik Mujibur,
Rahman Thahira,
Lakshmanaperumalsamy Perumalsamy,
Banat Ibrahim M.
Publication year - 2002
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/1521-4028(200208)42:4<284::aid-jobm284>3.0.co;2-m
Subject(s) - bioaugmentation , flavobacterium , micrococcus , microbial consortium , acinetobacter , biodegradation , diesel fuel , bacteria , corynebacterium , food science , pseudomonas , gasoline , contamination , biology , microbiology and biotechnology , bioremediation , bacillus (shape) , chemistry , microorganism , ecology , genetics , organic chemistry
Microbial enumeration and identification were carried out on several oil contaminated soil samples collected from gasoline and diesel stations. Bacteria were the most dominant microbiota and were therefore classified to generic level. Eleven main genera were detected and Corynebacterium was the predominant genus in all the samples. Biochemical characterisation and substrate utilisation showed high percentage of lipolytic ability combined with high inorganic nitrogen utilisers. The ability of these cultures to degrade crude oil was tested individually and in mixed bacterial consortium at different temperatures and pH values. Maximum crude oil biodegradation of 78% was achieved using a bacterial consortium containing five cultures ( Micrococcus sp. GS2‐22, Corynebacterium sp. GS5‐66, Flavobacterium sp. DS5‐73, Bacillus sp. DS6‐86 and Pseudomonas sp. DS10‐129) with 1% crude oil at 30 °C and pH 7.5. Such a consortium may be useful for bioaugmentation of oil contaminated environments.