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Molecular genotyping of isolates of Actinobacillus actinomycetemcomitans by pulsed‐field gel electrophoresis after separate digestion with Sse 8387I and Xho I
Author(s) -
Matsuda M.,
Takayama K.,
Haga S.,
Kagawa S.,
Ohta H.,
Fukui K.,
Moore J. E.
Publication year - 2002
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/1521-4028(200205)42:2<137::aid-jobm137>3.0.co;2-8
Subject(s) - pulsed field gel electrophoresis , xhoi , genotyping , biology , microbiology and biotechnology , genotype , serotype , gel electrophoresis , genomic dna , actinobacillus , restriction enzyme , genetics , dna , bacteria , gene , ecori
Genomic DNA from 18 Japanese clinical isolates of Actinobacillus actinomycetemcomitans was obtained from six periodontitis patients and analyzed using pulsed‐field gel electrophoresis (PFGE) after separate digestion with Sse 8387I and with Xho I. Three isolates from an identical patient were found to share an identical PFGE profile, and isolates from distinct patients were found to have PFGE profiles distinctly different from each other. Consequently, the 18 Japanese clinical isolates were discriminated into six distinct genotypes by means of PFGE. The genomic DNA from the other six reference strains (ATCC33384, ATCC43717, ATCC 43718, JCM2434, JCM2435 and Nig‐1) was discriminated into six genotypes by the same PFGE methodology, and these six genotypes were found to be distinctly different from the six genotypes of the 18 Japanese clinical isolates described above. Serotyping demonstrated three PFGE genotypes in the serotype a strains, four the serotype b strains and three the serotype c strains. The present results clearly suggest that the PFGE procedure after separate digestion with Sse 8387I and with Xho I has an excellent discriminatory power amongst strains and has a good genotypability for A. actinomycetemcomitans .