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Degradation of phenol by Trichosporon sp. LE3 cells immobilized in alginate
Author(s) -
Santos Vera L.,
Heilbuth Nadia M.,
Linardi Valter R.
Publication year - 2001
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/1521-4028(200107)41:3/4<171::aid-jobm171>3.0.co;2-c
Subject(s) - phenol , degradation (telecommunications) , calcium alginate , chemistry , chromatography , biodegradation , calcium , nuclear chemistry , organic chemistry , telecommunications , computer science
The degradation of phenol by freely suspended cells of Trichosporon sp. LE3 and alginate‐immobilized cells was studied in batch culture. The alginate concentration (2 or 4%) and the cross‐linking salt used (BaCl2 or CaCl2) affected the rate and percentage of phenol degradation. The highest values were obtained for immobilized cells at 2% calcium alginate, although complete degradation of 15 and 18 m M phenol was not observed. When the cell concentrations in the assays were doubled, the 2% calcium alginate‐immobilized cells were able to degrade up to 30 m M phenol in less than 120 hours, although the free cells did not completely degrade phenol at concentrations above 20 m M . The maximum phenol degradation rate was a strong function of initial phenol concentrations, being the highest values being observed for 20 m M phenol.