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Genetic diversity of Bacillus thuringiensis serovars revealed by RFLP using random DNA probes
Author(s) -
Joung KwangBo,
Lemaire Stéphan P.,
Côté JeanCharles
Publication year - 2001
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/1521-4028(200105)41:2<85::aid-jobm85>3.0.co;2-r
Subject(s) - ecori , biology , bacillus thuringiensis , restriction fragment length polymorphism , serotype , hindiii , phylogenetic tree , ribotyping , genetics , genetic diversity , dendrogram , microbiology and biotechnology , genotype , dna , restriction enzyme , bacteria , gene , demography , sociology , population
EcoRI and Hin dIII restriction fragment length polymorphism (RFLP) profiles using 2 random DNA probes, named 104 and 106, were generated for 85 B. thuringiensis strains. These include 80 serovars, 4 intra‐serovar strains: kurstaki HD‐1, dendrolimus , tenebrionis and sandiego , and a non‐serotypeable strain B. thuringiensis var. wuhanensis . A total of 47 Eco RI and 65 Hin dIII restriction patterns were generated when hybridization results from both probes were combined. Seventy‐seven B. thuringiensis strains showed distinctive hybridization profiles. The dendrogram resulting from the numerical analysis of the distance matrix revealed fourteen distinct phylogenetic groups at the 96% banding patterns similarity. The intra‐serovar strains showed higher similarity with their respective type serovars. However, different serovars from a common H‐serotype did not always cluster in the same phylogenetic group. Alternatively, several mosquitocidal serovars clustered in a single phylogenetic group. The correlation between serotyping and banding pattern similarity is discussed.