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Metabolism of benzonitrile by Cryptococcus sp. UFMG‐Y28
Author(s) -
Rezende Rachel Passos,
Teixeira Dias João Carlos,
Ferraz Vany,
Linardi Valter Roberto
Publication year - 2000
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/1521-4028(200012)40:5/6<389::aid-jobm389>3.0.co;2-j
Subject(s) - benzonitrile , chemistry , benzoic acid , organic chemistry
The yeast Cryptococcus sp. UFMG‐Y28 can utilize benzonitrile as a nitrogen and possible additional carbon source. The kinetics of growth on Yeast Carbon Base (YCB) added of benzonitrile as sole nitrogen source showed that benzonitrile was metabolized to benzoic acid and ammonia. Liquid chromatography analysis indicated that Cryptococccus sp. UFMG‐Y28 metabolized 12 mM benzonitrile to 10 m M benzoic acid. Resting cells cultivated on YCB‐propionitrile medium showed nitrilase activity against benzonitrile. This strain appears to be promising for bioconversion of nitriles to high value acids and for bioremediation of sites contaminated with aliphatic and aromatic nitriles.