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New enzymatic method for determining D ‐arabinitol in serum
Author(s) -
Hino Masayuki,
Tatsumi Noriyuki,
Yamane Takahisa,
Ohta Kensuke,
Takubo Takayuki,
Yabuuchi Masahiko
Publication year - 2000
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/1521-4028(200012)40:5/6<363::aid-jobm363>3.0.co;2-e
Subject(s) - oxidoreductase , mannitol , reagent , chemistry , enzyme , chromatography , nad+ kinase , biochemistry , organic chemistry
A new reagent has been developed to determine D ‐arabinitol. This utilizes D ‐arabinitol 2‐oxid‐oreductase derived from Bacillus sp. with high stability, and water‐soluble tetrazolium salt, that can detect NADH with high sensitivity. Since this enzyme does not react to D ‐mannitol, elimination of D ‐mannitol is unnecessary. Thus, this is a much simpler process than currently available with commercial kits use D ‐arabinitol 4‐oxidoreductase. The within‐run and between‐run precisions (CV) were 2.4–6.9% and 3.1–8.7%, respectively, whilst the correlation ( r ) between the results obtained with our proposed method ( y ) and those obtained with the commercial “Arabinitec‐auto” kit ( x ) was 0.964 ( y = 1.02 x + 0.933 μmol/l; n = 69). However, some samples deviated remarkably from correlation in both methods. Our analyzing accuracy is satisfactory in clinical application, as it does not miss positive sample over cut‐off value. We are refining this method by investigating why some specimens are apart from correlation significantly.