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Optimization of the cultivation medium for natamycin production by Streptomyces natalensis
Author(s) -
Farid Mohamed A.,
ElEnshasy Hesham A.,
ElDiwany Ahmed I.,
ElSayed ElSayed A.
Publication year - 2000
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/1521-4028(200007)40:3<157::aid-jobm157>3.0.co;2-1
Subject(s) - natamycin , yeast extract , food science , sodium nitrate , potassium nitrate , ammonium nitrate , phosphate , chemistry , ammonium , fermentation , antibiotics , potassium , nitrate , biomass (ecology) , nutrient , biology , biochemistry , agronomy , organic chemistry
The effects of certain nutrients on natamycin production by Streptomyces natalensis in submerged batch culture were studied. The production of this antibiotic required glucose in the cultivation medium with a concentration of 20 g/l. On the other hand, the highest antibiotic production was obtained in a cultivation medium containing 0.05 g/l of potassium dihydrogen phosphate. Further increase in phosphate concentration resulted in a significant increase in biomass concomitant with lower antibiotic production. Among different N‐sources tested, only ammonium sulphate, sodium nitrate and beef extract were the suitable nitrogen sources in supporting the antibiotic production. Furthermore, a mixture of beef extract and yeast extract (8 g/l and 2 g/l, respectively) exhibited a synergistic effect in enhancing the natamycin production reaching about 1.5 g/l.