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Molecularly imprinted polymer nanospheres as synthetic affinity receptors obtained by miniemulsion polymerisation
Author(s) -
Vaihinger Dorothea,
Landfester Katharina,
Kräuter Iris,
Brunner Herwig,
Tovar Günter E. M.
Publication year - 2002
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/1521-3935(200209)203:13<1965::aid-macp1965>3.0.co;2-c
Subject(s) - miniemulsion , molecular imprinting , molecularly imprinted polymer , polymer chemistry , dynamic light scattering , ethylene glycol dimethacrylate , polymer , emulsion polymerization , materials science , polymerization , chemical engineering , methacrylic acid , copolymer , chemistry , organic chemistry , nanoparticle , nanotechnology , selectivity , engineering , catalysis
Highly crosslinked polymer nanospheres composed of poly[(methacrylic acid)‐ co ‐(ethylene glycol dimethacrylate)] and poly(EGDMA) have been synthesised by miniemulsion polymerisation in presence of a chiral molecular template, L ‐ or D ‐Boc‐phenylalanine anilid. The miniemulsions and the resulting microgels were characterised by surface tension measurements, gravimetric analysis, dynamic light scattering, transmission electron microscopy, 1 H and 13 C CP‐MAS NMR, and Brunauer‐Emmett‐Teller gas adsorption measurements. The efficiency of the non‐covalent molecular imprinting was examined by binding experiments and quantified by UV absorption. The miniemulsions were converted to coagulate‐free and stable latexes with a conversion of 98 ± 2% and an apparent hydrodynamic particle diameter of 200 ± 20 nm. Molecular imprinting was most effective when a miniemulsion of molar ratio n MAA / n EGDMA = 0.25:1 was used for the microgel preparation. Enantioselective binding of the templates to the particles was observed. Binding of the L ‐enantiomer was four times greater in the L ‐imprinted polymer than in the non‐imprinted polymer and 10 times than binding of the D ‐enantiomer in the L ‐imprinted microgels. This new method allows for a one‐stage preparation of fully synthetic affinity receptors with a defined spherical shape and a high specific surface area due to their nanoscopic dimension.Transmission electron microscope photograph of particles of polymer 6 , prepared in presence of template.