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Dependence of water uptake and morphology of hyaluronan‐ and alginate‐based hydrogels on pH and degree of crosslinking
Author(s) -
Barbucci Rolando,
Consumi Marco,
Magnani Agnese
Publication year - 2002
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/1521-3935(200207)203:10/11<1292::aid-macp1292>3.0.co;2-i
Subject(s) - self healing hydrogels , carboxylate , polymer chemistry , chemistry , polysaccharide , scanning electron microscope , hydrogen bond , stoichiometry , morphology (biology) , iodide , infrared spectroscopy , chemical engineering , nuclear chemistry , molecule , organic chemistry , materials science , biology , engineering , genetics , composite material
Hydrogels of alginate and hyaluronan with 5%, 50% and 100% crosslinking, (percentage of polysaccharide carboxylate groups involved in crosslinking) are synthesized. Crosslinking and stoichiometry were varied by appropriate addition of chloromethylpyridine iodide (CMPI) to activate the carboxylic groups. Crosslinking percentage was determined by potentiometry, i.e., titrating the free carboxylate groups of the polysaccharide. Water uptake by the gels is studied in relation to crosslinking and pH. Hydrogen bonds in the three‐dimensional network, as shown by FT‐IR spectroscopy, are used to explain differences in water uptake and their dependence on pH. Scanning electron microscopy (SEM) analysis of the gels is performed at different pHs and the morphologies are compared with their chemical structures.Equilibrium water uptake of AA (gray) and HA (black) gels in relation to percentage crosslinking in 0.6 M NaCl.