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Mark–Houwink Parameters of Biosynthetic Poly( γ ‐glutamic acid) in Aqueous Solution
Author(s) -
Irurzun Isabel,
Bou Jordi J.,
PérezCamero Graciela,
Abad Concepción,
Campos Agustín,
MuñozGuerra Sebastián
Publication year - 2001
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/1521-3935(20011101)202:17<3253::aid-macp3253>3.0.co;2-c
Subject(s) - ionic strength , chemistry , gel permeation chromatography , aqueous solution , polymer chemistry , chromatography , phosphate buffered saline , analytical chemistry (journal) , intrinsic viscosity , polymer , organic chemistry
A combined viscosity–light scattering–gel permeation chromatography (GPC) study was carried out on bacterially produced poly( γ ‐glutamic acid) (PGGA). PGGA samples with weight‐average molecular weights ranging from 8×10 4 up to 8×10 5 g·mol –1 dissolved in phosphate buffer at 0.13 M ionic strength were used. It was found that the Mark–Houwink relation is acceptably obeyed, giving K and a values of 1.84×10 –6 dL·g –1 and 1.16, respectively. As expected, GPC analysis showed that PGGA does not follow the universal calibration plot and that deviations can not be avoided by modifying the ionic strength.