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Cholesterol Esterase Activity of a Molecularly Imprinted Polymer
Author(s) -
Kim JongMan,
Ahn KwangDuk,
Wulff Guenter
Publication year - 2001
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/1521-3935(20010401)202:7<1105::aid-macp1105>3.0.co;2-p
Subject(s) - chemistry , molecularly imprinted polymer , polymer , esterase , polymer chemistry , polymerization , amidine , phosphate buffered saline , molecular imprinting , organic chemistry , chromatography , selectivity , catalysis , enzyme
A molecularly imprinted polymer with cholesterol esterase activity was prepared. Polymerization of N , N ′‐diethyl(4‐vinylphenyl)amidine in the presence of the phosphate transition state analogue 2 followed by removal of the print molecule generated a catalytically active polymer with K m of 3.7 m M and k cat of 2.2·10 –4 min –1 , respectively. A relative rate enhancement of 27‐fold over the background buffer uncatalyzed reaction and of 2.4‐fold in the presence of the control polymer were observed.