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Detailed investigation on the molecular structure of carboxymethyl cellulose with unusual substitution pattern by means of an enzyme‐supported analysis
Author(s) -
Saake B.,
Horner S.,
Kruse Th.,
Puls J.,
Liebert T.,
Heinze Th.
Publication year - 2000
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/1521-3935(20001001)201:15<1996::aid-macp1996>3.0.co;2-x
Subject(s) - chemistry , carboxymethyl cellulose , fractionation , cellulose , hydrolysis , chromatography , polymer chemistry , organic chemistry , sodium
Four carboxymethyl cellulose samples derived from the synthesis concept of “reaction in reactive microstructures” were characterised in detail by combination of different analytical techniques. Fractionation of one of the samples according to water‐solubility into four fractions revealed that a non‐statistical distribution of the substituents occurred for all fractions. Endoglucanase fragmentation of three samples was performed followed by analytical and preparative SEC. The multi‐detected analytical SEC proved samples of high degree of substitution up to 1.9 to be intensively degraded, supporting the proposed block‐like substitution pattern. The detailed investigation of soluble fragmentation products by preparative SEC, hydrolysis and anion exchange chromatography with pulsed amperometric detection revealed that all samples contain fragments of much higher DS values than the average DS of the starting material. On the other hand, high quantities of degradation products of low DS or almost no substitution occurred. Therefore, a structure can be proposed with segments of very high DS alternating with areas of limited substitution. The result clearly showed that the high substituted fragments were dominated by 2,3,6‐tri‐ O ‐CMG.