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On‐line coupling of gradient‐HPLC and 1 H NMR for the analysis of random poly[(styrene)‐ co ‐(ethyl acrylate)]s
Author(s) -
Krämer Inge,
Hiller Wolf,
Pasch Harald
Publication year - 2000
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/1521-3935(20000901)201:14<1662::aid-macp1662>3.0.co;2-j
Subject(s) - copolymer , high performance liquid chromatography , tetrahydrofuran , ethyl acrylate , styrene , chemistry , acetonitrile , column chromatography , chromatography , proton nmr , acrylate , analytical chemistry (journal) , polymer chemistry , materials science , polymer , organic chemistry , solvent
Random poly[(styrene)‐ co ‐(ethyl acrylate)]s can effectively be analysed with respect to the chemical composition distribution by on‐line coupled HPLC/ 1 H NMR. The separation of the copolymers is conducted on a reversed‐phase column using acetonitrile/tetrahydrofuran as the eluent mixture. Via on‐line coupling, the chromatographic peaks are directly transferred into the NMR spectrometer and analysed on‐flow. Information on the chemical composition and the chemical heterogeneity of copolymers with high conversion is obtained. The experiments have been carried out using conventional HPLC‐grade solvents and no deuterium lock. The results of the on‐line HPLC/ 1 H NMR investigation have been correlated with a HPLC procedure based on calibration with narrow‐distributed copolymer standards.