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Effects of poloxamer on the gelation of silk fibroin
Author(s) -
Kang GyungDon,
Nahm JoongHee,
Park JongSin,
Moon JaeYu,
Cho ChongSu,
Yeo JooHong
Publication year - 2000
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/1521-3927(20000701)21:11<788::aid-marc788>3.0.co;2-x
Subject(s) - fibroin , poloxamer , crystallinity , poloxamer 407 , random coil , silk , materials science , infrared spectroscopy , chemical engineering , polymer chemistry , circular dichroism , polymer , chemistry , crystallography , copolymer , organic chemistry , composite material , engineering
The influence of the concentration of poloxamer 407, the pH and the temperature on the gelation of silk fibroin (SF) were studied. It was found that the gelation of SF occurred in the presence of poloxamer at pH value of 7.0 while gelation of SF itself did not occur. The gelation time of SF was shortened with increasing the poloxamer concentration and the temperature. The sol‐gel transition of SF became reversible with an addition of poloxamer. From infrared (IR) and circular‐dichroism (CD) spectroscopy measurements, it was found that a conformational change of the SF in the SF/poloxamer system from random coil to β‐structure was accelerated after forming a polymer complex with the poloxamer. The crystallinity of the poloxamer was reduced by SF from X‐ray diffraction measurements.