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Preparation of enzyme multilayers on colloids for biocatalysis
Author(s) -
Schüler Corinna,
Caruso Frank
Publication year - 2000
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/1521-3927(20000701)21:11<750::aid-marc750>3.0.co;2-3
Subject(s) - microelectrophoresis , biocatalysis , materials science , polystyrene , chemical engineering , immobilized enzyme , ethyleneimine , colloid , nanotechnology , adsorption , glucose oxidase , particle (ecology) , catalysis , chemistry , organic chemistry , enzyme , biosensor , chromatography , ionic liquid , polymer , composite material , electrophoresis , oceanography , geology , engineering
The construction of enzyme multilayer films on colloidal particles for biocatalysis is described. The enzyme multilayers were assembled on submicrometer‐sized polystyrene spheres via the alternate adsorption of poly(ethyleneimine) and glucose oxidase using a layer‐by‐layer approach. Microelectrophoresis and single particle light scattering measurements revealed regular and step‐wise assembly of the multilayers on the colloids. The high surface area bio‐multilayer coated particles formed were subsequently utilized in enzymatic catalysis.