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The Effects of Amino Acids on Production and Transport of α‐Amylase through Bacterial Membranes
Author(s) -
Ag?logğlu Sema,
Ensari N. Yavuz,
Uyar Fikret,
Otludil Birol
Publication year - 2000
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/1521-379x(20009)52:8/9<290::aid-star290>3.0.co;2-y
Subject(s) - membrane , amylase , chemistry , amino acid , biochemistry , food science , enzyme
Bacillus subtilis was grown in media containing various amino acids. The effects of these amino acids on the production of secreted α‐amylase and membrane‐bound α‐amylase were investigated. Their effects on the transport of α‐amylase through membranes were also determined. The synthesis rate of α‐amylase isolated from bacterial membranes was stimulated by growth in media separately containing Ala and Arg, all other amino acids being inhibitory. Cys, Arg and Leu increased the transport of α‐amylase through the membrane, while Ala, Asp and Ser inhibited it. Secreted α‐amylase activity was appreciably stimulated by Ala, Arg, Gln, Gly, Leu, Phe, Pro, and especially Cys while it was repressed by Asp, Asn, Glu, Lys, Met, Ser, Thr and Trp.