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Reporter Ribozymes for Real‐Time Analysis of Domain‐Specific Interactions in Biomolecules: HIV‐1 Reverse Transcriptase and the Primer–Template Complex
Author(s) -
Hartig Jörg S.,
Famulok Michael
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20021115)41:22<4263::aid-anie4263>3.0.co;2-7
Subject(s) - reverse transcriptase , ribozyme , primer (cosmetics) , human immunodeficiency virus (hiv) , computational biology , biomolecule , biology , virology , chemistry , rna , genetics , organic chemistry , gene
By fusing an aptamer to a ribozyme , an aptazyme was constructed (see picture) which sensitively and specifically detects the presence of HIV‐1 reverse transcriptase (RT). The fusion construct not only serves as a biosensor, but is also well suited for screening lead structures binding to the primer/template binding site of HIV‐1 RT.