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Pyrophosphate Detection in Water by Fluorescence Competition Assays: Inducing Selectivity through the Choice of the Indicator
Author(s) -
Fabbrizzi Luigi,
Marcotte Nathalie,
Stomeo Floriana,
Taglietti Angelo
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20021018)41:20<3811::aid-anie3811>3.0.co;2-w
Subject(s) - pyrophosphate , fluorescence , chemistry , competition (biology) , selectivity , receptor , biophysics , biochemistry , biology , ecology , enzyme , physics , quantum mechanics , catalysis
Displacement of the previously bound, and quenched , fluorescent indicator ( I ) by coordination of the pyrophosphate ion to the two Cu II centers of the receptor R results in the generation of the natural fluorescence of the indicator, and thus signals pyrophosphate recognition (see scheme). Discrimination from interfering anions (HPO 4 2− , SO 4 2− , NO 3 − , Cl − , NCO − , N 3 − , acetate, benzoate) can be achieved by tuning the indicator affinity towards the receptor.