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Efficient and Simple Solid‐Phase Synthesis of Short Cyclic Oligodeoxynucleotides Bearing a Phosphorothioate Linkage
Author(s) -
Smietana Michael,
Kool Eric T.
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20021004)41:19<3704::aid-anie3704>3.0.co;2-n
Subject(s) - phosphoramidite , oligonucleotide , solid phase synthesis , combinatorial chemistry , cleavage (geology) , chemistry , stereochemistry , materials science , dna , biochemistry , peptide , fracture (geology) , composite material
Only one additional step is required to extend oligonucleotide synthesis to generate cyclic oligonucleotides by standard automated phosphoramidite chemistry. After 5′‐iodination, cleavage from the solid support yields phosphorothioate‐bridged compounds 1 , which are under investigation as potentially bioactive molecules, in 40–80 % yields.