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A Molecular Beacon for Quantitative Monitoring of the DNA Polymerase Reaction in Real‐Time
Author(s) -
Summerer Daniel,
Marx Andreas
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20021004)41:19<3620::aid-anie3620>3.0.co;2-c
Subject(s) - molecular beacon , dna polymerase , real time polymerase chain reaction , dna , polymerase , chemistry , dna synthesis , microbiology and biotechnology , dna polymerase ii , polymerase chain reaction , biochemistry , biology , oligonucleotide , reverse transcriptase , gene
An assay for real‐time monitoring of DNA synthesis by DNA polymerases is presented. With the opening of a dual dye‐labeled probe, triggered by the progressing enzymatic DNA synthesis, a fluorescent signal is generated (see scheme), enabling the high‐throughput quantitative characterization of inhibitors and monitoring of the DNA polymerase reaction in cell lysates.