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Enzymatic Generation and In Situ Screening of a Dynamic Combinatorial Library of Sialic Acid Analogues
Author(s) -
Lins Roger J.,
Flitsch Sabine L.,
Turner Nicholas J.,
Irving Ed,
Brown Stuart A.
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020916)41:18<3405::aid-anie3405>3.0.co;2-p
Subject(s) - sialic acid , chemistry , aldolase a , in situ , enzyme , biochemistry , organic chemistry
Reversible formation of carbon–carbon bonds under physiological conditions by enzyme catalysis allows the generation and in situ screening of a dynamic mixture of biologically significant compounds. Generation of the dynamic library by incubation of the three sugars 1 a – c with two equivalents of sodium pyruvate in the presence of N ‐acetylneuraminic acid aldolase and wheat germ agglutinin resulted in amplification of sialic acid 1 a (see scheme).