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An Electrochemical Probe of DNA Stacking in an Antisense Oligonucleotide Containing a C3′‐ endo ‐Locked Sugar
Author(s) -
Boon Elizabeth M.,
Barton Jacqueline K.,
Pradeepkumar Pushpangadan I.,
Isaksson Johan,
Petit Catherine,
Chattopadhyaya Jyoti
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020916)41:18<3402::aid-anie3402>3.0.co;2-6
Subject(s) - stacking , dna , oligonucleotide , base pair , sugar phosphates , chemistry , nucleotide , a dna , duplex (building) , nucleobase , crystallography , biophysics , biochemistry , biology , gene , enzyme , organic chemistry
The preferred base‐stacking orientation of a conformationally constrained nucleotide (3′‐ endo‐ locked) within DNA/DNA and DNA/RNA duplexes (see picture) was probed by charge transport through DNA‐modified gold electrode surfaces. The conformation of the sugar is seen to sensitively determine the local stacking of the duplex. These results illustrate the utility of DNA‐mediated charge transport through DNA‐modified surfaces in characterizing small perturbations in DNA stacking and structure.