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Phosphite Dehydrogenase: A Versatile Cofactor‐Regeneration Enzyme
Author(s) -
Vrtis Jennifer M.,
White Andrea K.,
Metcalf William W.,
van der Donk Wilfred A.
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020902)41:17<3257::aid-anie3257>3.0.co;2-n
Subject(s) - cofactor , chemistry , enzyme , dehydrogenase , regeneration (biology) , deuterium , biochemistry , organic chemistry , biology , microbiology and biotechnology , physics , quantum mechanics
The highly thermodynamically favorable oxidation of phosphite to phosphate by phosphite dehydrogenase (PtxD) makes the enzyme useful for cofactor regeneration (see figure). Deuterium exchange in D 2 O provides labeled phosphite for the preparation of deuterated products.