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A Further Step towards Single‐Molecule Sequencing: Escherichia coli Exonuclease III Degrades DNA that is Fluorescently Labeled at Each Base Pair
Author(s) -
Brakmann Susanne,
Löbermann Sylvia
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020902)41:17<3215::aid-anie3215>3.0.co;2-6
Subject(s) - exonuclease , dna , escherichia coli , base pair , exonuclease iii , molecule , chemistry , single molecule real time sequencing , rhodamine , dna sequencing , biochemistry , dna polymerase , fluorescence , physics , gene , organic chemistry , dna sequencer , quantum mechanics
Single‐molecule sequencing comes into sight : exonuclease III of E. coli has been shown to perform the processive sequential hydrolysis of double‐stranded DNA with one strand being completely rhodamine‐labeled at each pyrimidine base. The performance of the exceptional enzyme was studied by steady‐state kinetic analysis based on quantitative fragment‐length determination.