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Fabrication of Carbohydrate Chips for Studying Protein–Carbohydrate Interactions
Author(s) -
Park Sungjin,
Shin Injae
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020902)41:17<3180::aid-anie3180>3.0.co;2-s
Subject(s) - carbohydrate , maleimide , chemistry , fluorescein , thiol , fabrication , biochemistry , nanotechnology , combinatorial chemistry , fluorescence , organic chemistry , materials science , physics , quantum mechanics , medicine , alternative medicine , pathology
A new tool for the high‐throughput study of carbohydrate–protein interactions: Maleimide‐linked carbohydrates (e.g. 1 ) were immobilized on thiol‐derivatized glass slides, and the microspots were probed with fluorescein‐labeled lectins (see picture). The binding of lectins to the carbohydrates on the slide depends on the concentration of the immobilized carbohydrates and on the length of the tethers.