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A Bacterial Small‐Molecule Three‐Hybrid System
Author(s) -
Althoff Eric A.,
Cornish Virginia W.
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020703)41:13<2327::aid-anie2327>3.0.co;2-u
Subject(s) - panning (audio) , dihydrofolate reductase , rna polymerase , protein subunit , computational biology , transfer rna , microbiology and biotechnology , repressor , rna , polymerase , biochemistry , biology , chemistry , gene , gene expression , paleontology , zoom , lens (geology)
A method for in vivo affinity chromatography , the yeast three‐hybrid assay simplifies protein identification and amplification at the end of affinity panning. The bacterial system described should increase, by several orders of magnitude, the number of protein variants that can be assayed (see picture; SLF = synthetic analogue of FK506; Mtx = methotrexate; FKBP12 = FK506‐binding protein 12; DHFR = dihydrofolate reductase; λcI = λ‐repressor; αNTD = N‐terminal domain of the α‐subunit of RNA Pol; RNA Pol = RNA polymerase).