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Fabricating Microarrays of Functional Proteins Using Affinity Contact Printing
Author(s) -
Renault Jean Philippe,
Bernard André,
Juncker David,
Michel Bruno,
Bosshard Hans Rudolf,
Delamarche Emmanuel
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020703)41:13<2320::aid-anie2320>3.0.co;2-z
Subject(s) - microcontact printing , protein microarray , materials science , substrate (aquarium) , lithography , fluorescence microscope , nanotechnology , contact print , soft lithography , fluorescence , planar , dip pen nanolithography , dna microarray , chemistry , optoelectronics , nanolithography , computer science , optics , biochemistry , computer graphics (images) , biology , composite material , fabrication , alternative medicine , ecology , gene expression , pathology , medicine , physics , gene
High‐resolution arrays of antibodies can be prepared in a highly parallel manner by a combination of affinity purification and microcontact printing. Arrays with lateral dimensions of between 100 and 3 μm were prepared by using planar, affinity stamps that were patterned by using various soft lithographic techniques. The fluorescence microscopy image shown demonstrates the placement of anti‐chicken and anti‐goat antibodies on a glass substrate from a stamp.