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Characterization of a Δ 8 ‐Sphingolipid Desaturase from Higher Plants: A Stereochemical and Mechanistic Study on the Origin of E , Z Isomers
Author(s) -
Beckmann Christoph,
Rattke Janine,
Oldham Neil J.,
Sperling Petra,
Heinz Ernst,
Boland Wilhelm
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020703)41:13<2298::aid-anie2298>3.0.co;2-g
Subject(s) - sphingolipid , olefin fiber , stereochemistry , chemistry , transformation (genetics) , yeast , double bond , biochemistry , gene , organic chemistry , catalysis
The simultaneous formation of E and Z double bonds results from the syn elimination of H and/or D atoms from different conformations of 4‐hydroxysphinganine [D 4 ] 1 . Δ 8 ‐Sphingolipid desaturase from Helianthus annuus is heterologously expressed in yeast and catalyzes the transformation to E olefin 2 (88%) and Z olefin 3 (12%).