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New Methods for Proteomic Research: Preparation of Proteins with N‐Terminal Cysteines for Labeling and Conjugation
Author(s) -
Tolbert Thomas J.,
Wong ChiHuey
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020617)41:12<2171::aid-anie2171>3.0.co;2-q
Subject(s) - protease , chemistry , cleavage (geology) , tobacco etch virus , biochemistry , combinatorial chemistry , terminal (telecommunication) , computational biology , enzyme , biology , virus , computer science , virology , telecommunications , paleontology , plant virus , potyvirus , fracture (geology)
The highly selective tobacco etch virus Nla protease (TEV protease) can be used to remove affinity tags with mutated cleavage sites to produce proteins for site‐specific labeling and chemoselective ligations (see scheme). This provides a simple, efficient method for producing proteins with N‐terminal cysteines for proteomic applications.