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Colorimetric and Fluorometric Detection of Nucleic Acids Using Cationic Polythiophene Derivatives
Author(s) -
Ho HoangAnh,
Boissinot Maurice,
Bergeron Michel G.,
Corbeil Geneviève,
Doré Kim,
Boudreau Denis,
Leclerc Mario
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020503)41:9<1548::aid-anie1548>3.0.co;2-i
Subject(s) - cationic polymerization , nucleic acid , chemistry , oligonucleotide , alkoxy group , polythiophene , fluorescence , combinatorial chemistry , polymer , stereochemistry , organic chemistry , dna , biochemistry , conductive polymer , alkyl , physics , quantum mechanics
Different electrostatic interactions and conformational structures between single‐ or double‐stranded negatively charged nucleic acids bound to a surface (see picture) and water‐soluble cationic poly(3‐alkoxy‐4‐methylthiophene) derivatives (in yellow) provide the basis for a sensitive optical detection method (colorimetric or fluorometric) for oligonucleotide hybridization.