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A Mass Spectrometry Based Direct‐Binding Assay for Screening Binding Partners of Proteins
Author(s) -
Zou Hanfa,
Zhang Qingchun,
Guo Zhong,
Guo Baochuan,
Zhang Qiang,
Chen Xiaoming
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020215)41:4<646::aid-anie646>3.0.co;2-i
Subject(s) - mass spectrometry , chemistry , chromatography , plasma protein binding , ionization , binding site , desorption , analytical chemistry (journal) , biochemistry , adsorption , organic chemistry , ion
Simple and versatile : In a new assay for screening binding partners of proteins (see schematic representation), the target protein is first immobilized on a porous silicon probe, which is then incubated with a mixture of the possible binding partners. The captured binding partners are identified on‐probe by means of laser desorption/ionization time‐of‐flight mass spectrometric analysis.