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Protein Affinity Labeling Mediated by Genetically Encoded Peptide Tags
Author(s) -
Amini Frank,
Kodadek Thomas,
Brown Kathlynn C.
Publication year - 2002
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20020118)41:2<356::aid-anie356>3.0.co;2-m
Subject(s) - biotinylation , tripeptide , glycine , peptide , library science , histidine , chemistry , computational biology , biochemistry , computer science , amino acid , biology
A useful method for identifying the binding partners of a given protein: The complex of Ni II with the tripeptide NH 2 ‐glycine‐glycine‐histidine or the hexahistidine peptide mediates biotinylation of proximal proteins through an oxidatively triggered reaction (see scheme).