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Direct Microscopic Observation of the Time Course of Single‐Molecule DNA Restriction Reactions
Author(s) -
Schäfer Bürk,
Gemeinhardt Helgard,
Greulich Karl Otto
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20011217)40:24<4663::aid-anie4663>3.0.co;2-j
Subject(s) - dna , restriction enzyme , molecule , biophysics , chemistry , fluorescence microscope , fluorescence , sticky and blunt ends , biology , biochemistry , physics , optics , organic chemistry
A slice of life : Individual, fluorescently labeled DNA molecules are cut by a restriction enzyme into a sequence‐specific fingerprint‐like pattern and the time course of the reaction is analyzed by fluorescence microscopy. As shown in the picture, all the cuts occur one after another from one end to the other along the DNA molecule.