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A High‐Throughput‐Screening Method for the Identification of Active and Enantioselective Hydrolases
Author(s) -
Baumann Markus,
Stürmer Rainer,
Bornscheuer Uwe T.
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20011119)40:22<4201::aid-anie4201>3.0.co;2-v
Subject(s) - microtiter plate , enantioselective synthesis , acetic acid , high throughput screening , chromatography , hydrolase , chemistry , enzyme , identification (biology) , combinatorial chemistry , biochemistry , catalysis , biology , botany
A rapid and reliable test for the determination of hydrolase activity and enantioselectivity comprises the conversion of acetic acid released from acetates to NADH by using a commercially available enzymatic test‐kit (see scheme). The NADH is spectrophotometrically quantified in a microtiter plate format.