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On‐Line Liquid Chromatography Mass Spectrometry: A Useful Tool for the Detection of DNA Sequence Variation
Author(s) -
Oberacher Herbert,
Oefner Peter J.,
Parson Walther,
Huber Christian G.
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20011015)40:20<3828::aid-anie3828>3.0.co;2-w
Subject(s) - mass spectrometry , chromatography , variation (astronomy) , line (geometry) , dna , chemistry , analytical chemistry (journal) , physics , mathematics , astrophysics , geometry , biochemistry
Indications of the occurrence of mutations can be gained from accurate mass measurements by electrospray ionization mass spectrometry. On‐line coupling to liquid chromatography is essential for the application to real samples. By utilizing the coupled method, insertions, deletions, and point mutations are rapidly discovered in DNA restriction fragments and PCR products (see image).