Premium
DNA‐Directed Functionalization of Colloidal Gold with Proteins
Author(s) -
Niemeyer Christof M.,
Ceyhan Bülent
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20011001)40:19<3685::aid-anie3685>3.0.co;2-e
Subject(s) - colloidal gold , biotinylation , dna , surface modification , conjugate , streptavidin , nanotechnology , chemistry , covalent bond , nanoparticle , combinatorial chemistry , materials science , biochemistry , biotin , organic chemistry , mathematical analysis , mathematics
A supramolecular construction kit comprises DNA‐functionalized gold nanoparticles, covalent DNA–streptavidin conjugates, and biotinylated antibodies and allows the generation of functional nanoparticle reagents (see schematic illustration). The combination of specific antibodies and DNA‐stabilized colloidal gold is applicable for the detection of antigens attached to a chip.