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Hapten‐Functionalized DNA‐Streptavidin Nanocircles as Supramolecular Reagents in a Competitive Immuno‐PCR Assay
Author(s) -
Niemeyer Christof M.,
Wacker Ron,
Adler Michael
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010903)40:17<3169::aid-anie3169>3.0.co;2-i
Subject(s) - streptavidin , biotinylation , hapten , chemistry , immunoassay , supramolecular chemistry , dna , analyte , combinatorial chemistry , oligonucleotide , reagent , conjugate , biotin , biochemistry , antibody , chromatography , biology , molecule , organic chemistry , mathematical analysis , mathematics , immunology
Analysis with nanorings : The endogeneous proteins of supramolecular DNA nanocircles, obtained in high yields from oligomeric precursors containing bisbiotinylated DNA and streptavidin, are conveniently functionalized with biotinylated hapten moieties (see schematic representation). These modular conjugates can be used as reagents in a novel competitive immunoassay for the high‐sensitivity detection of low molecular weight analytes.