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Highly Sensitive Novel Biosensor Based on an Immobilized lac Repressor
Author(s) -
Bontidean Ibolya,
Kumar Ashok,
Csöregi Elisabeth,
Galaev Igor Yu.,
Mattiasson Bo
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010716)40:14<2676::aid-anie2676>3.0.co;2-5
Subject(s) - inducer , repressor , biosensor , lac repressor , schematic , chemistry , biophysics , molecule , dna , operator (biology) , biochemistry , biology , transcription factor , gene , engineering , organic chemistry , electronic engineering
Sensitive repressors : The conformational change that occurs in the lac repressor protein when it binds to an inducer molecule has been used to develop a biosensor that permits the detection of the corresponding operator sequence or specific inducer molecules. A capacitive signal transducer was used to translate the conformational change of the lac repressor protein, covalently immobilized on a gold electrode (see schematic representation, A=bound inducer molecule or DNA), into a measurable signal.