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Electronic Transduction of Polymerase or Reverse Transcriptase Induced Replication Processes on Surfaces: Highly Sensitive and Specific Detection of Viral Genomes
Author(s) -
Patolsky Fernando,
Lichtenstein Amir,
Kotler Moshe,
Willner Itamar
Publication year - 2001
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/1521-3773(20010618)40:12<2261::aid-anie2261>3.0.co;2-p
Subject(s) - reverse transcriptase , transduction (biophysics) , polymerase , dna , dna polymerase , biology , microbiology and biotechnology , genome , oligonucleotide , rolling circle replication , chemistry , rna , biophysics , biochemistry , gene
Replication, precipitation, and amplification : Polymerase or reverse transcriptase induced replication of DNA/RNA on a transducer (electrode or piezoelectric crystal) leads to the ultrasensitive specific electronic transduction of viral genomes. Biotin tags (B) on the double‐stranded assembly provide docking sites for a conjugate between avidin (A) and an alkaline phosphatase (AP). Enzyme biocatalysis of substrate (S) to the insoluble product (P), which precipitates onto the transducer (yellow surface), provides amplification in the analysis of the target DNA.